Author: Geoffrey Rolls

From patient to pathologist, preparing tissue specimens for histological examination requires care, skill and sound procedures. This straightforward guide to good histology practice provides practical advice on best-practice techniques and simple ways to avoid common errors. Each aspect of the histology process is covered:

  • Specimen collection
  • Grossing
  • Tissue processing
  • Paraffin embedding
  • Sectioning and microtomes
  • Routine staining (H&E)
  • Special stains
  • IHC staining (immunohistochemistry)
  • ISH staining (in situ hybridization).

Each of the 101 steps provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

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